<i>S</i>-Adenosyl-<scp>L</scp>-methionine Activates Actinorhodin Biosynthesis by Increasing Autophosphorylation of the Ser/Thr Protein Kinase AfsK in<i>Streptomyces coelicolor</i>A3(2)
-
- JIN Ying-Yu
- Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University
-
- CHENG Jinhua
- Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University
-
- YANG Seung Hwan
- Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University
-
- MENG Lingzhu
- Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University
-
- PALANIYANDI Sasikumar Arunachalam
- Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University
-
- ZHAO Xin-Qing
- Department of Bioscience and Bioengineering, Dalian University of Technology
-
- SUH Joo-Won
- Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University
書誌事項
- タイトル別名
-
- S-Adenosyl-L-methionine Activates Actinorhodin Biosynthesis by Increasing Autophosphorylation of the Ser/Thr Protein Kinase AfsK in Streptomyces coelicolor A3(2)
この論文をさがす
抄録
S-Adenosyl-L-methionine (SAM) is one of the major methyl donors in all living organisms. The exogenous treatment with SAM leads to increased actinorhodin production in Streptomyces coelicolor A3(2). In this study, mutants from different stages of the AfsK-AfsR signal transduction cascade were used to test the possible target of SAM. SAM had no significant effect on actinorhodin production in afsK, afsR, afsS, or actII-open reading frame 4 (ORF4) mutant. This confirms that afsK plays a critical role in delivering the signal generated by exogenous SAM. The afsK-pHJL-KN mutant did not respond to SAM, suggesting the involvement of the C-terminal of AfsK in binding with SAM. SAM increased the in vitro autophosphorylation of kinase AfsK in a dose-dependent manner, and also abolished the effect of decreased actinorhodin production by a Ser/Thr kinase inhibitor, K252a. In sum, our results suggest that SAM activates actinorhodin biosynthesis in S. coelicolor M130 by increasing the phosphorylation of protein kinase AfsK.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 75 (5), 910-913, 2011
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282681455227264
-
- NII論文ID
- 10028272608
-
- NII書誌ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 11088665
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可