Quantitative Comparison of Adipocytokine Gene Expression during Adipocyte Maturation in Non-obese and Obese Rats
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- Ikeda Yoshito
- Department of Biophysical Chemistry, Kyoto Pharmaceutical University
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- Hama Susumu
- Department of Biophysical Chemistry, Kyoto Pharmaceutical University
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- Kajimoto Kazuaki
- Laboratory of Innovative Nanomedicine, Graduate School of Pharmaceutical Sciences, Hokkaido University
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- Okuno Takashi
- Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
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- Tsuchiya Hiroyuki
- Department of Biophysical Chemistry, Kyoto Pharmaceutical University
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- Kogure Kentaro
- Department of Biophysical Chemistry, Kyoto Pharmaceutical University
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抄録
Adipocytokines secreted from adipocytes have been extensively analyzed due to their role as key factors in various complications of obesity, including arterial sclerosis, liver steatosis, insulin resistance, and diabetes. Several in vivo and in vitro studies have suggested that adipocyte maturation is related to fluctuations in adipocytokine secretion. However, the relationship between adipocyte maturation and adipocytokine levels has not been fully elucidated. Therefore, we sought to clarify the link between adipocytokine gene expression and adipocyte maturation through systematic analysis. We quantified mRNA for six adipocytokine genes: adiponectin, resistin, leptin, plasminogen activator inhibitor 1 (PAI-1), heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF), and visfatin, in adipose tissue, in primary cultured adipocytes obtained from an obese Zucker rat, and in the preadipocyte cell line 3T3-L1. Moreover, to elucidate the role of adipocytokines in adipocyte maturation, adipocytokine expression levels were analyzed during maturation. Although fluctuations in adipocytokine gene expression were heterogeneous, gene expression was highly similar during maturation of primary cultured adipocytes from obese and non-obese rats, suggesting that the maturation process is independent from processes that lead to obesity. Moreover, the expression patterns of adiponectin, resistin and leptin mRNA in 3T3-L1 cells were highly similar to those in primary cultured adipocytes, indicating that these adipocytokines could be common maturation markers for primary cultured adipocytes obtained from obese and non-obese rats, and for preadipocyte cell lines.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 34 (6), 865-870, 2011
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679611170560
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- NII論文ID
- 130000738062
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 11095270
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可