Cornuside Suppresses Lipopolysaccharide-Induced Inflammatory Mediators by Inhibiting Nuclear Factor-Kappa B Activation in RAW 264.7 Macrophages

• Choi Yun Ho

  Department of Anatomy, Medical School, Institute for Medical Sciences, Chonbuk National University

• Jin Guang Yu

  Yanbian University Hospital, Medicine College, Yanbian University

• Li Guang Zhao

  Department of Anatomy and Histology and Embryology, School of Basic Medical Sciences, Yanbian University

• Yan Guang Hai

  Department of Anatomy and Histology and Embryology, School of Basic Medical Sciences, Yanbian University

抄録

Cornuside, a secoiridoid glucoside compound, was isolated from the fruit of Cornus officinalis SIEB. et ZUCC. Cornuside has been reported to possess immunomodulatory and anti-inflammatory activities. However, the effects and mechanism of action of cornuside in inflammation have not been fully characterized. The present study was therefore designed to examine whether cornuside suppresses inflammatory response in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Cornuside significantly inhibited the LPS-induced production of nitric oxide, prostaglandin E₂, tumor necrosis factor-alpha, interleukin-6 (IL-6), and IL-1beta. The mRNA and protein expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were also decreased by cornuside. Furthermore, cornuside significantly attenuated the LPS-stimulated phosphorylation and degradation of inhibitory kappa B-alpha and the subsequent translocation of the p65 subunit of nuclear factor-kappa B (NF-κB) to the nucleus. Cornuside also reduced the phosphorylations of extracellular-signal-related kinase (ERK1/2), p38, and c-Jun N-terminal kinase (JNK1/2). These results suggest that the anti-inflammatory property of cornuside is related to the downregulations of iNOS and COX-2 due to NF-κB inhibition as well as the negative regulation of ERK1/2, p38, and JNK1/2 phosphorylations in RAW 264.7 cells.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 34 (7), 959-966, 2011

  公益社団法人 日本薬学会

キーワード

• cornuside
• lipopolysaccharide
• inducible nitric oxide synthase
• cyclooxygenase-2
• nuclear factor-kappa B

詳細情報

• CRID: 1390001204632723200
• NII論文ID: 130000738081
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.34.959
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 11132357
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11132357
• Web Site: http://www.jstage.jst.go.jp/article/bpb/34/7/34_7_959/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012151798
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可