ウズラオボトランスフェリンの細胞傷害特性

• 小田嶋 真里

  岐阜大学大学院連合農学研究科(信州大学)

• 大谷 元

  信州大学農学部

書誌事項

タイトル別名

• Studies on Cytotoxic Properties of Japanese Quail Ovotransferrin

抄録

Cytotoxic properties of Japanese quail ovotransferrin were estimated by trypan blue exclusion. The ovotransferrin revealed a cytotoxicity toward C3H/HeN mouse T-lymphocytes, mouse B-lymphocytes and Jurkat Clone E6-1 cells (a human cell line derived from acute leukemia T-lymphocyte), while it was inert to C3H/HeN mouse plastic-adherent cells and Japanese quail spleen cells. DNA fragmentation was observed on terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling and agarose gel electrophoresis when mouse spleen lymphocytes were cultured with quail ovotransferrin for 12h. On the other hand, the cytotoxic activity of ovotransferrin diminished completely when the protein was treated with PNGase F or pepsin, whereas a strong cytotoxic activity remained after tryptic digestion of iron-saturated quail ovotransferrin. A cytotoxic glycoprotein having molecular weights of 39, 000 was obtained from the trypsin digest. These results indicate that Japanese quail ovotransferrin may induce apoptosis to mouse spleen T-and B-lymphocytes and Jurkat cells, but not to quail spleen lymphocytes and mouse monocytes/macrophages. Moreover, these results suggest that the cytotoxic activity is attributable to C-lobe in quail ovotransferrin.

収録刊行物

• 日本畜産学会報

  日本畜産学会報 71 (9), 338-346, 2000

  公益社団法人 日本畜産学会

キーワード

• Quail ovotransferrin
• Cytotoxicity
• Lymphocytes
• Apoptosis
• Glycopeptide

詳細情報

• CRID: 1390001205194103296
• NII論文ID: 130000742460
• DOI: 10.2508/chikusan.71.9_338
• ISSN: 18808255
• ISSN: 1346907X
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可