牛骨格筋メトミオグロビン還元酵素のNADH-チトクロムb₅還元酵素としての同定

• 有原 圭三

  北里大学獣医畜産学部

• 伊藤 良

  北里大学獣医畜産学部

• 近藤 洋

  北里大学獣医畜産学部

書誌事項

タイトル別名

• Identification of Bovine Skeletal Muscle Metmyoglobin Reductase as an NADH-Cytochrome b₅ Reductase
• ウシ コッカクキン メト ミオグロビン カンゲン コウソ ノ NADH チトク

抄録

Metmyoglobin reductase was purified from bovine skeletal muscle by procedures including affinity chromatography on a 5'-AMP-Sepharose 4B to an electrophoretically homogeneous protein. The enzyme had been purified over 20, 000 fold, and it has a pH optimum of 6.5. Molecular weight was estimated at 33, 000. Isoelectric pH was between 5.6-5.8. The enzyme reduced metmyoglobin in the presence of erythrocyte cytochrome b₅. A Km for cytochrome b₅ was 3.2×10-6M. These properties of the purified metmyoglobin reductase were similar to those of NADH-cytochrome b₅ reductase purified from a bovine erythrocyte. Both enzymes were flavoproteins. Immunological studies have indicated that metmyoglobin reductase is indistinguishable from the erythrocyte enzyme. It was concluded, therefore, that bovine skeletal muscle metmyoglobin reductase was identical molecularly to erythrocyte NADH-cytochrome b₅ reductase.

収録刊行物

• 日本畜産学会報

  日本畜産学会報 60 (1), 46-56, 1989

  公益社団法人 日本畜産学会

キーワード

• metmyoglobin reductase
• NADH-cytochrome b₅ reductase
• bovine
• muscle
• erythrocyte

詳細情報

• CRID: 1390282680171809024
• NII論文ID: 130000751595
• NII書誌ID: AN00195188
• DOI: 10.2508/chikusan.60.46
• ISSN: 18808255
• ISSN: 00215309
• ISSN: 1346907X
• NDL書誌ID: 3224770
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3224770
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可