天然由来物質バイカレインによるmitogen-activated protein kinase(MAPK)カスケードの抑制

• 中畑 則道

  東北大学大学院薬学研究科分子生物薬学教室

• 姜 れいき

  東北大学大学院薬学研究科分子生物薬学教室

• 久津輪 瑞代

  東北大学大学院薬学研究科分子生物薬学教室

• 大泉 康

  東北大学大学院薬学研究科分子生物薬学教室

書誌事項

タイトル別名

• Inhibition of mitogen-activated protein kinase cascade by baicalein, a flavonoid of natural origin
• テンネン ユライ ブッシツ バイカレイン ニ ヨル mitogen activated protein kinase MAPK カスケード ノ ヨクセイ

抄録

Mitogen-activated protein kinase (MAPK) is a family of serine/threonine kinase that appears to be a component common to signalling pathway initiated by a wide range of factors including hormones, differentiation factors and mitogens. Baicalein is a flavonoid derived from the root of Scutellaria baicaleins. From searching the inhibitors of prostaglandin synthesis in C6 rat glioma cells, we found that baicalein had a potent inhibitory activity of prostaglandin synthesis induced by either histamine or A23187, a Ca²⁺ ionophore. Baicalein inhibited histamine- and A23187-induced phosphorylation of MAPK in the cells, which was known to cause the phosphorylation of cytosolic phospholipase A₂. Baicalein also inhibited the phosphorylation of MAPK kinase (MEK) induced by histamine or A23187 in the cells. In vitro kinase assay, baicalein inhibited the activities of raf but not MEK. These results imply that baicalein is a specific inhibitor of MAPK cascade, acting on phosphorylation of MEK by raf.

収録刊行物

• 日本薬理学雑誌

  日本薬理学雑誌 114 (supplement), 215-219, 1999

  公益社団法人 日本薬理学会

キーワード

• バイカレイン
• プロスタグランジンE<SUB>2</SUB>
• MAPK
• MEK
• raf

詳細情報

• CRID: 1390001204270951680
• NII論文ID: 130000754545
• NII書誌ID: AN00198335
• DOI: 10.1254/fpj.114.supplement_215
• ISSN: 13478397
• ISSN: 00155691
• NDL書誌ID: 4912704
• Web Site: http://id.ndl.go.jp/bib/4912704
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4912704
• Web Site: https://search.jamas.or.jp/link/ui/2000094337
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可