Effects of lyophilization and storage of rat liver microsome on activity of aniline hydroxylase,contents of cytochrome b5 and cytochrome P-450 and aniline-induced P-450 difference spectrum

• KAMATAKI Tetsuya

  Department of Biochemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Chiba

• KITAGAWA Haruo

  Department of Biochemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Chiba

書誌事項

タイトル別名

• EFFECTS OF LYOPHILIZATION AND STORAGE OF RAT LIVER MICROSOMES ON ACTIVITY OF ANILINE HYDROXYLASE, CONTENTS OF CYTOCHROME b<SUB>5</SUB> AND CYTOCHROME P-450 AND ANILINE-INDUCED P-450 DIFFERENCE SPECTRUM
• EFFECTS OF LYOPHILIZATION AND STORAGE OF RAT LIVER MICROSOMES ON ACTIVITY OF ANILINE HYDROXYLASE, CONTENTS OF CYTOCHROME b5 AND CYTOCHROME P-450 AND ANILINE-INDUCED P-450 DIFFERENCE SPECTRUM

抄録

Effects of lyophilization and storage of rat liver 9, 000×g supernatant fraction or microsomes on activity of aniline hydroxylase, contents of cyt. P-450 and cyt. b₅ and aniline-induced P-450 difference spectrum were studied. The lyophilization of rat liver 9, 000×g supernatant fraction or microsomes produced about 20% decrease in the activity of aniline hydroxylase. Aniline hydroxylase was stably stored for 6 months if lyophilized 9, 000×g supernatant fraction of rat liver had been stored in an atmosphere of nitrogen or in a low pressure of air. Activity of aniline hydroxylase and contents of cyt. P-450 and cyt. b₅ were not reduced during aerobical storage for a week. When lyophilized microsomes were stored for 6 months anaerobically, aniline hydroxylase, cyt. P-450 and cyt. b₅ were more stable as compared to aerobical storage. However, activity of aniline hydroxylase was reduced approx. 40% by the storage. The reduction of aniline hydroxylase activity was more significant than that of contents of cyt. P-450 and cyt. b₅. Reduction of either activity of aniline hydroxylase or contents of cyt. P-450 and cyt. b₅ was more marked when lyophilized microsomes were stored with glycerol. Aniline hydroxylase in lyophilized 9, 000×g supernatant fraction from phenobarbital treated rabbit was stably stored, and aerobically little activity was lost during storage for 7 months.

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 24 (2), 195-203, 1974

  公益社団法人 日本薬理学会

キーワード

• Pharmacology

詳細情報

• CRID: 1390001204288277376
• NII論文ID: 130000838342
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.24.195
• COI: 1:CAS:528:DyaE2cXlsVOgtL4%3D
• ISSN: 13473506
• ISSN: 00215198
• ISSN: http://id.crossref.org/issn/00215198
• NDL書誌ID: 7673331
• PubMed: 4546794
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7673331
• Web Site: https://search.jamas.or.jp/link/ui/1974112146
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可