Inhibitory Effects of Hyaluronan on (14C)Arachidonic Acid Release from Labeled Human Synovial Fibroblasts.

• Tobetto Kenji

  Research Laboratories, Maruho Co., Ltd.

• Yasui Tadashi

  Research Laboratories, Maruho Co., Ltd.

• Ando Takao

  Research Laboratories, Maruho Co., Ltd.

• Hayaishi Masahiro

  Hayaishi Hospital

• Motohashi Noriko

  Department of Radiopharmaceutical Chemistry, Kobe Women''s College of Pharmacy

• Shinogi Masaki

  Department of Radiopharmaceutical Chemistry, Kobe Women''s College of Pharmacy

• Mori Itsuhiko

  Department of Radiopharmaceutical Chemistry, Kobe Women''s College of Pharmacy

抄録

The effects of hyaluronan (HA) on the release of arachidonic acid (AA) from phospholipids induced by bradykinin in synovial fibroblasts of osteoarthritic patients were examined. HA inhibited [¹⁴C]AA release from prelabeled synovial cells stimulated with and without bradykinin 1 hr after incubation with HA and thereafter. The inhibitory effects of HA on [¹⁴C]AA release were dependent on the concentration and molecular weight of HA. However, inhibition of [¹⁴C]AA release by HA was not merely due to the viscosity of HA. The [¹⁴C]AA release induced by calcium-ionophore A23187 was also inhibited by HA with a high molecular weight. In addition, HA did not affect [¹⁴C]AA uptake by the cells. Our results suggest that HA with a high molecular weight elicits anti-inflammatory effects, at least in part, by inhibiting AA release in inflamed joints.

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 60 (2), 79-84, 1992

  公益社団法人 日本薬理学会

キーワード

• Hyaluronan
• Arachidonic acid release
• Synovial cell

詳細情報

• CRID: 1390282679264381312
• NII論文ID: 130000840142
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.60.79
• COI: 1:CAS:528:DyaK38XmsVGhsrg%3D
• ISSN: 13473506
• ISSN: 00215198
• ISSN: http://id.crossref.org/issn/00215198
• PubMed: 1479746
• Web Site: https://search.jamas.or.jp/link/ui/1994188371
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可