Heparin Inhibits the Progression Phase of Subcultured Endothelial Cell Proliferation in Rat Aorta.

Kimura Ikuko, Nagaura Takeshi, Naitoh Takeshi, Kobayashi Shinjiro, Kimura Masayasu

doi:10.1254/jjp.60.369

The anti-proliferative effects of heparin on subcultured endothelial cells (EC) of rat aorta were investigated to determine whether heparin inhibits the competence phase or the progression phase in the cell cycle using the starting time and the rate of proliferation. Fetal bovine serum (FBS)-stimulated EC proliferation increased to 1.5-times in cell number, but decreased to 0.5-times in ³H-thymidine incorporation, compared with the proliferation of rat primary cultured smooth muscle cells (SMC). The FBS-effects on EC proliferation were attributed to the progression phase rather than the competence phase. Heparin (1, 10 or 100 μg/ml) significantly inhibited the proliferation of FBS (5%)-stimulated EC, and the extent of inhibition was the same in both cell number and ³H-thymidine incorporation. In the ³H-thymidine incorporation every 3 hr, heparin reduced the rate of incorporation into G₀-arrested EC, but did not affect the starting time of DNA synthesis. When the index of competence phase, starting time, was plotted against that of progression phase, rate of proliferation, the inhibition of heparin was attributed to the progression phase. These results demonstrate that heparin selectively inhibits the progression phase in subcultured EC of rat aorta.

Heparin Inhibits the Progression Phase of Subcultured Endothelial Cell Proliferation in Rat Aorta.

この論文をさがす

抄録

収録刊行物

被引用文献 (5)*注記

参考文献 (32)*注記

キーワード

詳細情報詳細情報について

書き出し

問題の指摘

Heparin Inhibits the Progression Phase of Subcultured Endothelial Cell Proliferation in Rat Aorta.

この論文をさがす

抄録

収録刊行物

被引用文献 (5)*注記

参考文献 (32)*注記

キーワード

詳細情報 詳細情報について

書き出し

問題の指摘

参加プロジェクトリスト

詳細情報詳細情報について