Effects of Stilbene Derivatives SITS and DIDS on Development of Intracellular Acidosis during Ischemia in Isolated Guinea Pig Ventricular Papillary Muscle In Vitro.

• Lai Zhong-Fang

  <I>Department of Pharmacology, Kumamoto University School of Medicine</I>

• Liu Jie

  <I>Department of Pharmacology, Kumamoto University School of Medicine</I>

• Nishi Katsuhide

  <I>Department of Pharmacology, Kumamoto University School of Medicine</I>

書誌事項

タイトル別名

• Effects of Stilbene Derivatives SITS an

抄録

Using ion-selective microelectrode techniques, we investigated the effects of 4-acetamido-4''-isothiocyanatostilbene-2, 2''-disulfonic acid (SITS) and 4, 4''-diisothiocyanostilbene-2, 2''-disulfonic acid (DIDS), which are known as Cl¯-HC0₃¯ exchange blockers, on action potentials and intracellular pH (pH_i) in guinea pig ventricular papillary muscles subjected to simulated ischemia. Simulated ischemia was produced by stopping the flow of superfusing solution and then covering the preparations with mineral oil. Simulated ischemia induced a progressive decrease in the maximum upstroke rate and resting membrane potentials, shortened action potential duration, and resulted in cessation of action potentials within 10minus;12 min after the onset of simulated ischemia. The pH_i-measurements revealed progressive intracellular acidosis during the period of simulated ischemia. SITS (0.5 mM) or DIDS (O.1 mM) delayed the onset of ischemia-induced deterioration of action potentials and prolonged the time to cessation of action potentials. SITS or DIDS (0.1minus;0.5 mM) induced an increase in pH_i in HC0₃¯-buffered solution and suppressed the development of intracellular acidosis during ischemia. Under the external Cl¯-free condition, the time to cessation of action potentials caused by ischemia was significantly delayed, and the development of intracellular acidosis during ischemia was attenuated. The present results indicate that activation of the Cl¯-HC0₃¯ exchange system would be involved, in part, in the development of intracellular acidosis during cardiac ischemia.

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 72 (2), 161-174, 1996

  公益社団法人 日本薬理学会

キーワード

• Cl¯-HC0<SUB>3</SUB>¯ exchange
• Simulated cardiac ischemia
• Intracellular pH
• Stilbene derivative
• Ion-selective microelectrode

詳細情報

• CRID: 1390282679264014976
• NII論文ID: 130000841232
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.72.161
• COI: 1:CAS:528:DyaK28XmsVCrtrs%3D
• ISSN: 13473506
• ISSN: 00215198
• ISSN: http://id.crossref.org/issn/00215198
• NDL書誌ID: 4071310
• PubMed: 8912917
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4071310
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819339253?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819339253?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/1997125564
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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