Nucleotide Sequence of eCG α-Subunit cDNA and Its Expression in the Equine Placenta
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- MIN Kwan-Sik
- Laboratories of Cellular Biochemistry
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- SHINOZAKI Mikihiko
- Laboratories of Cellular Biochemistry
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- MIYAZAWA Kiyoshi
- Laboratory of Theriogenology, Obihiro University
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- NISHIMURA Ryohei
- Surgery, Veterinary Medical Science/Animal Resource Science, The University
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- SASAKI Nobuo
- Surgery, Veterinary Medical Science/Animal Resource Science, The University
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- SHIOTA Kunio
- Laboratories of Cellular Biochemistry
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- OGAWA Tomoya
- Laboratories of Cellular Biochemistry
書誌事項
- タイトル別名
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- Nucleotide Sequence of eCG .ALPHA.-Subunit cDNA and Its Expression in the Equine Placenta.
- Nucleotide Sequence of eCG アルファ Subunit
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Equine chorionic gonadotropin (eCG) is a member of the glycoprotein family, which includes lutropin (LH), follitropin (FSH), and thyrotropin (TSH), all consisting of two noncovalently linked a-and β-subunits. In the present study, we determined the full length of the equine α-subunit cDNA sequence. mRNA was extracted from equine placenta on day 70 of gestation. The nucleotide sequence of the eCG a-subunit cDNA was amplified by PCR using mixed primers designed on the basis of the nucleotide sequence of human, rat and mouse a-subunits. Analysis of the nucleotide sequence revealed that the a-subunit amplified contained 387 bp which included the signal peptide (24 amino acids containing 4 amino acids expected) and the first 3 amino acid residues. When the expression of both subunit mRNAs in the equine placenta was compared by Northern blotting, the expression of the β-subunit mRNA was relatively greater than that of the a-subunit.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 40 (4), 301-305, 1994
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206333426176
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- NII論文ID
- 130000846181
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- NII書誌ID
- AA10936678
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- COI
- 1:CAS:528:DyaK2MXkvF2lurc%3D
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- ISSN
- 13484400
- 09168818
- http://id.crossref.org/issn/09168818
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- NDL書誌ID
- 3603840
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可