Ｘ線照射により誘発した染色体損傷に対する茶カテキンの抑制効果

• 杉澤 彩子

  独立行政法人国立健康・栄養研究所

• 梅垣 敬三

  独立行政法人国立健康・栄養研究所

• 山田 和彦

  独立行政法人国立健康・栄養研究所

書誌事項

タイトル別名

• Protective Effect of Tea Catechin against X-ray-induced Chromosomal Damage
• Xセン ショウシャ ニ ヨリ ユウハツ シタ センショクタイ ソンショウ ニ タイスル チャ カテキン ノ ヨクセイ コウカ

抄録

We examined the protective effect of tea catechin against X-ray-induced chromosomal damage using the micronucleus assay. Male ICR mice were orally administered tea catechin extract at 10-300mg/kgBW/day for 7 days, then subjected to X-ray irradiation at 0.5 Gy. The resulting chromosomal damage to bone marrow was evaluated by the micronucleus assay using peripheral blood. Tea catechin extract tended to protect the bone marrow from X-ray-induced chromosomal damage. To examine this radioprotective effect of tea catechin in more detail, human lymphoblastoid WIL2-NS cells were incubated with various concentrations of (-) -epigallocatechin3-O-gallate (EGCg), the main constituent of tea catechins, and then exposed to 0.5 Gy of X-ray radiation. The resulting chromosomal damage was evaluated by a cytokinesis-block micronucleus assay. A high dose of EGCg (10μM) protected WIL2-NS cells from X-ray-induced chromosomal damage, but a physiological dose (<1μM) did not. EGCg was markedly decomposed by X-ray irradiation, indicating a direct reaction between EGCg and X-rays. These results suggest that although tea catechin has a radioprotective effect, no such effect can be expected at a physiological dose.

収録刊行物

• 日本栄養・食糧学会誌

  日本栄養・食糧学会誌 56 (2), 85-90, 2003

  公益社団法人 日本栄養・食糧学会

キーワード

• カテキン
• 染色体損傷
• X線

詳細情報

• CRID: 1390001206294061056
• NII論文ID: 130000856371
• NII書誌ID: AN00311992
• DOI: 10.4327/jsnfs.56.85
• ISSN: 18832849
• ISSN: 02873516
• NDL書誌ID: 6561296
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6561296
• Web Site: https://search.jamas.or.jp/link/ui/2003232363
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可