マダイイリドウイルスの物理化学的性状と細胞感受性

  • 中島 員洋
    National Research Institute of Aquaculture, Fisheries Agency
  • 反町 稔
    National Research Institute of Aquaculture, Fisheries Agency

書誌事項

タイトル別名
  • Biological and Physico-chemical Properties of the Iridovirus Isolated from Cultured Red Sea Bream, Pagrus major.
  • マダイイリドウイルスの物理化学的性状と細胞感受性〔英文〕
  • マダイイリド ウイルス ノ ブツリ カガクテキ セイジョウ ト サイボウ カン
  • Biological and physic-chemical properties of the iridovirus isolated from cultured red sea bream, Pagrus major

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抄録

Eleven fish cell lines were compared for susceptibility to red sea bream iridovirus (RSIV). Cytopathic effect was produced in BF-2, CHSE-214, FHM, JSKG, KRE-3, RTG-2 and YTF cell lines among the cell lines examined. The titers of the virus on BF-2 and KRE-3 were higher than those on the other cell lines.The pretreatment of BF-2 and KRE-3 cells with polyethylene glycol (PEG) or addition of PEG to the sample increased the virus titers. RSIV replicated at 15°C, 20°C, 25°C and 30°C but not at 37°C. The suitable temperature for viral growth was 20°C or 25°C. The virus was sensitive to acid (pH 3), chloroform and ether and unstable to heat but stable to ultrasonic treatment and repeated freezing and thawing. Treatment with 5-iodo-2-deoxyuridine (IUdR) reduced the titers of the virus.

収録刊行物

  • 魚病研究

    魚病研究 29 (1), 29-33, 1994

    日本魚病学会

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