腹水症を呈するブリ稚魚から分離されたウイルスについて

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  • Characteristics and pathogenicity of a virus isolated from yellowtail fingerlings showing ascites.
  • フクスイショウ オ テイスル ブリ チギョ カラ ブンリサレタ ウイルス ニ

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In early summer, 1983, an acute disease characterized by ascites occurred among yellowtail fingerlings, Seriola quinqueradiata, cultured in a farm in the Seto Inland Sea, Japan, and accordingly virological investigations were carried out in July.<BR>All three inoculants prepared from the viscera of the fish with ascites produced cytopathic effects (CPE) of pyknosis on RTG-2, CHSE-214 and EK-1 cells within 1.5-5 days after inoculation at 20°C. The virus replicated in CHSE-214 and EK-1 cells at temperature between 5-30°C. Electron micrographs revealed pentagonal or hexagonal particles of 62-69 nm. The virus was resistant to pH 3, pH 11, ether and chloroform and was stable at 56°C for 30 minutes. Replication of the virus was not inhibited by 5-Iodo-2'-deoxyuridine. Neutralization tests with antisera proved that this virus resembled to IPNV.<BR>Pathogenicity of the virus was studied using yellowtail fingerlings (average body weight 1.1 g). Fish were exposed to the virus by immersion for 1 hour in seawater containing106.6 TCID50 /ml of the virus. The fish infected with the virus showed mortality of 62 % at water temperature of 20°C and 14 % at 25°C, while controls showed mortality of only 4 % at 20°C and 10% at 25°C, respectively. Moribund fish infected with the virus were often accompanied by severe ascites and hemorrhage in the liver. The virus reisolated from all the infected fish died.<BR>The results strongly suggest that this virus was the causative agent of the ascites of yellowtail fingerlings. The agent was tentatively named yellowtail ascites virus (YAV).

収録刊行物

  • 魚病研究

    魚病研究 19 (4), 231-238, 1985

    日本魚病学会

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