Characterization of Colony-Stimulating Activity in a Medium Conditioned by a CSA Producing Cell Line

DOI Web Site 被引用文献1件
  • KIMURA Nobuhiro
    The First Department of Internal Medicine, Faculty of Medicine, Kyushu University
  • NIHO Yoshiyuki
    The First Department of Internal Medicine, Faculty of Medicine, Kyushu University
  • YANASE Toshiyuki
    The First Department of Internal Medicine, Faculty of Medicine, Kyushu University

抄録

A human cell line (KONT) producing colony-stimulating activity (CSA) has been established.1) The KONT cell line was isolated from the pleural effusion of a lung cancer patient who had developed marked leukocytosis. The cells produce both human CSA and mouse CSA. Medium conditioned by KONT cells is much more potent in stimulating human bone marrow cell growth than is human leukocyte conditioned medium (HL-CM). Furthermore, the KONT conditioned medium tends to induce granulocytic cells rather than other cell types. The CSA is stable at temperatures ranging from 37°C to 50°C, but is completely inactivated at a temperature of 80°C for 30min. The CSA is also sensitive to digestion by trypsin and pronase, and there is little binding of mouse or human CSA in KONT- CM to concanavalin A-Sepharose. Fractionation of the conditioned medium by Sephadex G-150 chromatography indicated that the maximum activity of the CSA for human marrow cells is eluted at 20, 000-28, 000 dalton fraction.

収録刊行物

被引用文献 (1)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ