Identificatioin of the α isoform of preprotachykinin-A mRNA in synovial fibroblasts isolated from patients with rheumatoid arthritis

• ABE Kazuki

  Research Laboratory, Minophagen Pharmaceutical Co.

• NISHIKAWA Takuji

  Department of Orthopedic Surgery, University of Tokyo

• YAMAMOTO Seizou

  Department of Orthopedic Surgery, Tokyo Metropolitan Geriatric Medical Hospital

• KOSHIHARA Yasuko

  Department of Nutrition, Tokyo Metropolitan Institute of Gerontology

• HIRABAYASHI Kazuhiro

  Research Laboratory, Minophagen Pharmaceutical Co.

• SATO Toshitsugu

  Research Laboratory, Minophagen Pharmaceutical Co.

• INOUE Hideo

  Research Laboratory, Minophagen Pharmaceutical Co.

書誌事項

タイトル別名

• Identification of the α isoform of preprotachykinin-A mRNA in synovial fibroblasts isolated from patients with rheumatoid arthritis
• Identificatioin of the アルファ isoform of preprotachykinin A mRNA in synovial fibroblasts isolated from patients with rheumatoid arthritis

抄録

Previous study showed that synovial fibroblasts derived from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) express β-preprotachykinin (PPT)-A mRNA and release substance P (SP). In this study, we examined the presence of an isoform of PPT-A mRNA in synovial and normal skin fibroblasts by reverse transcription-polymerase chain reaction (RT-PCR) analysis. RA synovial fibroblasts, but not OA synovial and skin fibroblasts, expressed α-isoform PPT-A mRNA together with β-PPT-A mRNA. However, expression of γ- and δ-PPT mRNAs was not observed in synovial and skin fibroblasts. Levels of both α- and β-PPT-A mRNAs were increased in RA fibroblasts treated with transforming growth factor (TGF)-β and basic fibroblast growth factor (bFGF). Treatment with TGF-β and bFGF strongly increased the expression of α- and β-PPT-A mRNAs compared to the individual treatment. Peak levels of α-PPT-A mRNA were reached at 8h whereas those of β-PPT-A mRNA were maximal at 12h after treatment with TGF-β and bFGF. These findings suggest that SP production by synovial fibroblasts in RA is regulated through signaling pathways that lead to the generation of both α- and β-PPT-A mRNAs.

収録刊行物

• Ｐｒｏｃｅｅｄｉｎｇｓ　ｏｆ　ｔｈｅ　Ｊａｐａｎ　Ａｃａｄｅｍｙ．　Ｓｅｒ．　Ｂ：　Ｐｈｙｓｉｃａｌ　ａｎｄ　Ｂｉｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ

  Ｐｒｏｃｅｅｄｉｎｇｓ　ｏｆ　ｔｈｅ　Ｊａｐａｎ　Ａｃａｄｅｍｙ．　Ｓｅｒ．　Ｂ：　Ｐｈｙｓｉｃａｌ　ａｎｄ　Ｂｉｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ 77 (10), 203-207, 2001

  日本学士院

キーワード

• Substance P
• synovial fibroblasts
• RT-PCR
• rheumatoid arthritis
• osteoarthritis
• preprotachykinin-A mRNA

詳細情報

• CRID: 1390001204147355648
• NII論文ID: 130000907979
• NII書誌ID: AA00785485
• DOI: 10.2183/pjab.77.203
• ISSN: 13492896
• ISSN: 03862208
• NDL書誌ID: 6048797
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6048797
• Web Site: http://www.jstage.jst.go.jp/article/pjab1977/77/10/77_10_203/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2002170260
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可