Endocytosis of azurophil granules released from neutrophils by junctional epithelial cells of rat gingiva.

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  • AYASAKA N.
    First Department of Oral Anatomy Faculty of Dentistry, Kyushu University
  • KONDO T.
    First Department of Oral Anatomy Faculty of Dentistry, Kyushu University
  • NAGATA E.
    Department of Pedodontics, Kyushu University
  • TANAKA T.
    First Department of Oral Anatomy Faculty of Dentistry, Kyushu University

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Abstract

A cytochemical study using diaminobenzidine (DAB) reaction and horseradish peroxidase (HRP) tracer or DAB reaction alone was performed on junctional epithelium (JE) of young (32-to 42-day-old) and old (100-day-old) rats to investigate how JE cells endocytose the azurophil granules released from neutrophils, as JE cells do with HRP.<BR>HRP was endocytosed by JE cells, and the endocytosed HRP products fused with one another or cytoplasmic vacuoles and formed phagolysosomes of amorphous shape, which digestion of the HRP seemed to be progressive. It was thus certified that JE cells possess endocytotic ability. In old rat gingivae incubated with only DAB medium, neutrophils were found in the JE and contained endogenous peroxidasepositive round, ovoid and elongated azurophil granules. In particular, at the coronal portion of JE, endogenous peroxidase-positive round ovoid and elongated reaction products were numerously detected in the intercellular spaces of the JE cells and within the JE cells. Judging from the cytochemical and morphological resemblances between the endogenous peroxidase-positive reaction products and the azurophil granules in the neutrophils, the reaction products in the intercellular spaces corresponded to the azurophil granules released from neutrophils, and those in the JE cells corresponded to the azurophil granules endocytosed by the JE cells. Furthermore, the endogenous peroxidase-positive reaction products endo cytosed by JE cells also formed the same amorphous phagolysosomes as formed by HRP. It was, therefore, concluded that, at the coronal portion of the JE of old rat gingivae, the JE cells may endocytose and digest lysosomes (azurophil granules) released from neutrophils to protect the gingival tissue.

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