Effect of Hypoxia Mimetic Cobalt Chloride on the Expression of Extracellular-Superoxide Dismutase in Retinal Pericytes

• Adachi Tetsuo

  Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University

• Aida Kazunari

  Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University

• Nishihara Hiroko

  Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University

• Kamiya Tetsuro

  Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University

• Hara Hirokazu

  Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University

抄録

The initial clinical stage of diabetic retinopathy (DR) is characterized by the development of intraretinal microvascular abnormalities. The increased formation of reactive oxygen species (ROS) is thought to be a key event in the pathogenesis of DR. Extracellular-superoxide dismutase (EC-SOD) is an anti-inflammatory enzyme that is distributed mainly in vascular cells and protects cells from ROS by scavenging superoxide anion. Treatment with cobalt chloride (CoCl₂) decreased the expression of EC-SOD but not other SOD isozymes in pericytes accompanied with an increase of intracellular ROS production. Pre-treatment with N-acetylcysteine (NAC) significantly suppressed the ROS production and down-regulation of EC-SOD. We observed the activation of caspase-3 and DNA fragmentation as signs of apoptotic process by CoCl₂ treatment. In addition, these phenomena were significantly inhibited by pre-treatment with NAC. EC-SOD enhancer 4-phenyl butyric acid also suppressed the caspase-3 activation. It is known that the presence of a high level of EC-SOD throughout the vessel walls might have an important protective role against superoxide in the vascular system. The decrease in EC-SOD expression accompanied with elevation of ROS level in pericytes under hypoxia might induce and/or promote the ROS-triggered apoptosis of pericytes and the development of pathogenesis in DR.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 34 (8), 1297-1300, 2011

  公益社団法人 日本薬学会

キーワード

• extracellular-superoxide dismutase
• hypoxia
• apoptosis
• diabetic retinopathy
• reactive oxygen species

詳細情報

• CRID: 1390001204632739584
• NII論文ID: 130000936602
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.34.1297
• COI: 1:STN:280:DC%2BC3Mjis1WjtA%3D%3D
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 11174801
• PubMed: 21804221
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11174801
• Web Site: http://www.jstage.jst.go.jp/article/bpb/34/8/34_8_1297/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012183431
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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