Distribution of Dendritic Cells in Normal Human Salivary Glands

• Le An

  Division of Pediatric Dentistry, Department of Craniofacial Sciences, University of Connecticut School of Dental Medicine

• Saverin Michele

  Division of Pediatric Dentistry, Department of Craniofacial Sciences, University of Connecticut School of Dental Medicine

• Hand Arthur R.

  Division of Pediatric Dentistry, Department of Craniofacial Sciences, University of Connecticut School of Dental Medicine

抄録

Dendritic cells (DC) are believed to contribute to development of autoimmune sialadenitis, but little is known about their distribution in normal salivary glands. In this study, DC were identified and their distribution was determined in normal human parotid and submandibular glands. For light microscopy, salivary gland sections were stained with H&E or immunocytochemically using antibodies to DC markers. Transmission electron microscopy (TEM) was used to evaluate the ultrastructural characteristics of DC. In H&E sections, elongated, irregularly shaped nuclei were occasionally seen in the striated and excretory duct epithelium. Immunolabeling with anti-HLA-DR, anti-CD11c and anti-S100 revealed DC with numerous processes extending between ductal epithelial cells, often close to the lumen. Morphometric analyses indicated that HLA-DR-positive DC occupied approximately 4–11% of the duct wall volume. Similar reactive cells were present in acini, intercalated ducts and interstitial tissues. TEM observations revealed cells with indented nuclei containing dense chromatin, pale cytoplasm with few organelles, and lacking junctional attachments to adjacent cells. These results indicate that DC are abundant constituents of normal human salivary glands. Their location within ductal and acinar epithelium suggests a role in responding to foreign antigens and/or maintaining immunological tolerance to salivary proteins.<br>

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 44 (4), 165-173, 2011

  日本組織細胞化学会

キーワード

• antigen presenting cells
• immunocytochemistry
• electron microscopy
• parotid gland
• submandibular gland

詳細情報

• CRID: 1390282679837569536
• NII論文ID: 130000941527
• DOI: 10.1267/ahc.11010
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc/44/4/44_11010/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012244414
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • CiNii Articles
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