-
- MATSUZAKI KENZO
- First Department of Internal Medicine, Faculty of Medicine, The University of Tokyo
-
- MIYAZAKI TATSUO
- First Department of Internal Medicine, Faculty of Medicine, The University of Tokyo
-
- YASUDA KIKUO
- Teikoku Hormone Mfg, Co., Ltd.
この論文をさがす
抄録
A6 cells, a continuous cell line derived from kidney of Xenopus laevis, were incubated with [3H]-dexamethasone for 24h. When radioactive compounds in media were separated by reversed phase high pressure liquid chromatography, two radioactive fractions were found. The less polar fraction which contained 91-93% of total radioactivity cochromatographed with dexamethasone, whereas the polar fraction contained 5% of total radioactivity in media. In order to rigorously identify the polar metabolite, large scale cultures were carried out and the polar compound was separated and purified by reversed phase high pressure liquid chromatography. The purified material was analyzed by secondary ion mass spectrometry and nuclear magnetic resonance spectroscopy. By these procedures, this material was identified as 6β-hydroxydexamethasone.<BR>To our knowledge these are the first data indicating that dexamethasone can be metabolized by transporting epithelia such as A6 cells.
収録刊行物
-
- Endocrinologia Japonica
-
Endocrinologia Japonica 38 (2), 131-135, 1991
一般社団法人 日本内分泌学会