Thyroid Microsomal Antigen in Graves' Thyroid is Not Different from That in Normal Thyroid.
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- HAMADA NOBORU
- Thyroid Study Unit, Sumire Hospital, Osaka Social Welfare Foundation
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- DEGROOT LESLIE J.
- Thyroid Study Unit, Department of Medicine, University of Chicago
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- PORTMANN LUC
- Thyroid Study Unit, Department of Medicine, University of Chicago
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- YAMAKAWA JUNICHI
- Second Department of Internal Medicine, Osaka City University Medical School
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- NOH JAEDUK
- Ito Thyroid Clinic and Hospital
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- OKAMOTO YASUYUKI
- Second Department of Internal Medicine, Osaka City University Medical School
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- OHNO MARIKO
- Second Department of Internal Medicine, Osaka City University Medical School
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- ITO KUNIHIRO
- Ito Thyroid Clinic and Hospital
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- MORII HIROTOSHI
- Second Department of Internal Medicine, Osaka City University Medical School
抄録
Differences from normal in microsomal antigen (M-Ag) may be involved in the development of autoimmune thyroid disease. We compared the M-Ag in Graves' thyroid immunologically and biochemically to that in normal thyroid. The concentration of M-Ag, measured with an enzyme-linked immunosorbent assay, was significantly greater in the Graves' microsomes than in normal microsomes. Binding of a patient's microsomal antibody to Graves' microsomes was completely inhibited when the serum was first incubated with normal thyroid microsomes. Sodium dodecylsulfate-polyacrylamide gel electrophoresis and Western blotting were done with a monoclonal antibody to denatured M-Ag. In both Graves' and normal thyroids, M-Ag existed as 107-, 101-, and 95-kDa peptides. After incubation with V8 protease, the residual antigenic peptide had a molecular weight of less than 60, 000 and after incubation with trypsin, 95- and 87-kDa peptides and several smaller antigenic peptides were found. There were no significant differences in the pattern of normal and Graves' microsomes after digestion. Twodimensional gel electrophoresis of Graves' microsomes showed that the isoelectric point for the 107-kDa peptide was at pH 7.2; that for the 101-kDa peptide was at pH 6.2, and that for the 95-kDa peptide was at 6.5. These values were not different from those observed for normal microsomes. These results indicate that M-Ag in Graves' thyroid does not differ from that in normal thyroid, and that microsomal antibodies in autoimmune thyroid disease probably do not arise from differences in the antigen.
収録刊行物
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- Ｅｎｄｏｃｒｉｎｏｌｏｇｉａ Ｊａｐｏｎｉｃａ
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Ｅｎｄｏｃｒｉｎｏｌｏｇｉａ Ｊａｐｏｎｉｃａ 38 (5), 471-478, 1991
一般社団法人 日本内分泌学会
