cDNA Cloning of Androgen-Repressed mRNA in Rat Seminal Vesicles: Partial Characterization of a cDNA Clone, pSvr-1.

DOI Web Site PubMed
  • IZAWA MASAO
    Division of Physiology, Institute of Steroid Research, Tottori University School of Medicine

この論文をさがす

抄録

When the in vitro translation products of mRNAs from castrated animals (48h) were compared with those from androgen-treated animals (48 h) to survey the molecular mechanism of androgen-responsive gene expressions in the rat seminal vesicles, some peptide bands which were repressed in response to androgen were observed. From these findings, we constructed a partial cDNA library of poly (A+) RNAs which had been isolated from the seminal vesicles of castrated rats (48 h) and modestly enriched with respect to the concentration of androgen-repressed mRNAs by sucrose density gradient centrifugation, and screened by differential colony hybridization. One cDNA clone, pSvr-1, whose mRNA is markedly induced within 24h after castration of the animal in the seminal vesicles as well as in the ventral prostate, was isolated. pSvr-1 hybridized to a mRNA of 1, 700 nucleotides in length. Partial sequence analysis showed that this clone had highly homologous but not identical sequences to those reported for rat sulfated glycoprotein-2. This cDNA clone may provide a useful probe for the study of the negative regulation mechanism of gene expression by androgens.

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ