アサガオのラクトース耐性および非耐性株における生理的差異

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  • 久島 繁
    筑波大学応用生物化学系代謝化学研究室 キャルガリー大学生物学科
  • 渡辺 恒夫
    筑波大学応用生物化学系代謝化学研究室
  • 新井 勇治
    筑波大学応用生物化学系代謝化学研究室
  • THORPE Trevor A.
    Plant Physiology Research Group, Department of Biology, University of Calgary

書誌事項

タイトル別名
  • Physiological Differences between Lactose-adapted and Non-adapted Cells of Japanese Morning-glory
  • アサガオ ノ ラクトース タイセイ オヨビ ヒ タイセイ カブ ニ オケル セ

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抄録

To study the mechanism of lactose adaptation, several trials were carried out using original non-adapted and lactose-adapted callus cultures of Japanese morning-glory (Pharbitis nil L. var Violet). In both types of cells the activities of lactase, UDPgalppase, UDPGppase and UDPgal epimerase were present. Non-adapted cells thus appeared to be potentially capable of lactose metabolism. However, UDPgal epimerase activity in these cells was very low and cell wall lactase activity was about 30% of that of the lactose-adapted cells. When lactose-adapted cells were cultured on lactose medium, the glucose content remained low and galactose high throughout the culture period. Thus, it appears that these cells preferentially utilized the glucose moiety over galactose. Contents of G-6-P, G-1-P and Gal-1-P in galactose-grown original cells increased about 3 times over those in sucrose-grown original cells. UDPG and UDPgal contents increased about 1.5 times and only marginally, respectively, over those of sucrose-grown original cells. In comparison, the contents of G-6-P, G-1-P and Gal-1-P in galactose-grown lactose-adapted cells increased about two times over those of sucrose-grown original cells and decreased by about 20% of those of galactose-grown original cells. This suggests that the adapted cells have a greater capacity to metabolize lactose and galactose than the non-adapted cells. Based on these results, inhibition by lactose and galactose, and adaptation to galactose and lactose are discussed.

収録刊行物

  • 植物組織培養

    植物組織培養 2 (2), 52-58, 1985

    日本植物細胞分子生物学会

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