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- 吉岡 健太郎
- 名古屋大学医学部第3内科
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- 各務 伸一
- 名古屋大学医学部第3内科
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- 藤 明彦
- 名古屋大学医学部第3内科
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- 田原 裕文
- 名古屋大学医学部第3内科
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- 村瀬 賢一
- 名古屋大学医学部第3内科
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- 鵜浦 雅志
- 金沢大学医学部第1内科
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- PRIMUS F. James
- Department of Medicine, New Jersey Medical School
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- LEEVY Carroll M.
- Department of Medicine, New Jersey Medical School
書誌事項
- タイトル別名
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- Production of monoclonal anti-Mallory body antibody.
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抄録
To elucidate the nature of the non-keratin component of Mallory bodies (MBs), monoclonal anti-Mallory body antibody, anti-NMB-3, was prepared using cell fusion technique. Anti-NMB-3 reacted with MBs in the liver but not with intermediate filaments (IFs) of normal human liver or skin. Anti-NMB-3 reacted with MBs of alcoholic hepatitis, Wilson's disease, primary biliary cirrhosis, fatty liver of diabetes mellitus, hepatocellular carcinoma, and griseofulvin-treated mice under immunofluorescence microscopy. In western blotting, anti-NMB-3 reacted with the bands of molecular weight (MW) 42 and 70-92 kd of MBs separated by SDS-polyacrylamide gel electrophoresis, while polyclonal anti-keratin antibody reacted with the bands of MW 45, 55, 62, and 66 kd. These results indicate that anti-NMB-3 reacts with a non-keratin component of MBs. Anti-NMB-3 may be useful for the study of the nature of MBs, and immunofluorescence microscopy with anti-NMB-3 may be a good tool for detecting MBs in liver specimens.
収録刊行物
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- 日本消化器病学会雑誌
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日本消化器病学会雑誌 84 (6), 1251-1257, 1987
一般財団法人 日本消化器病学会