meso-Dihydroguaiaretic Acid Inhibits Hepatic Lipid Accumulation by Activating AMP-Activated Protein Kinase in Human HepG2 Cells

• Lee Myoung-Su

  Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University

• Kim Kyung Jin

  Department of Biomaterials Science and Engineering, Yonsei University

• Kim Daeyoung

  Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University

• Lee Kyung-Eun

  Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University

• Hwang Jae-Kwan

  Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University Department of Biomaterials Science and Engineering, Yonsei University Translational Research Center for Protein Function Control, Yonsei University

抄録

Hepatic lipid accumulation is a major risk factor for dyslipidemia, nonalcoholic fatty liver disease, and insulin resistance. The present study was conducted to evaluate hypolipidemic effects of meso-dihydroguaiaretic acid (MDA), anti-oxidative and anti-inflammatory compound isolated from the Myristica fragrans HOUTT., by oil red O staining, reverse transcription-polymerase chain reaction (RT-PCR), and Western blot. MDA significantly inhibited insulin-induced hepatic lipid accumulation in a dose-dependent manner. The lipid-lowering effect of MDA was accompanied by increased expression of proteins involved in fatty acid oxidation and decreased expression of lipid synthetic proteins. In addition, MDA activated AMP-activated protein kinase (AMPK) as determined by phosphorylation of acetyl-CoA carboxylase (ACC), a downstream target of AMPK. The effects of MDA on lipogenic protein expression were suppressed by pretreatment with compound C, an AMPK inhibitor. Taken together, these findings show that MDA inhibits insulin-induced lipid accumulation in human HepG2 cells by suppressing expression of lipogenic proteins through AMPK signaling, suggesting a potent lipid-lowering agent.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 34 (10), 1628-1630, 2011

  公益社団法人 日本薬学会

キーワード

• <i>meso</i>-dihydroguaiaretic acid
• HepG2 cell
• hepatic lipid accumulation
• AMP-activated protein kinase

詳細情報

• CRID: 1390001204631327232
• NII論文ID: 130001086599
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.34.1628
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 11250232
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11250232
• Web Site: http://www.jstage.jst.go.jp/article/bpb/34/10/34_10_1628/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012139298
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可