Effects of a Purified Saponin Mixture from Alfalfa on Plasma Lipid Metabolism in Hyperlipidemic Mice

• Yu Cong-Hong

  School of Pharmaceutical Sciences, Sun Yat-sen University

• Xie Guo

  Zhongshan Institute, University of Electronic Science and Technology of China Institute of Traditional Chinese Medicine and Natural Products, Jinan University

• He Rong-Rong

  Institute of Traditional Chinese Medicine and Natural Products, Jinan University

• Zhai Yu-Jia

  Institute of Traditional Chinese Medicine and Natural Products, Jinan University

• Li Yi-Fang

  Institute of Traditional Chinese Medicine and Natural Products, Jinan University

• Tsoi Bun

  Institute of Traditional Chinese Medicine and Natural Products, Jinan University

• Kurihara Hiroshi

  Institute of Traditional Chinese Medicine and Natural Products, Jinan University

• Yang De-Po

  School of Pharmaceutical Sciences, Sun Yat-sen University

抄録

Alfalfa (Medicago sativa L.) is a genus distributed widely throughout the world and considered as the richest land source of nature products. In this study, we obtained a purified saponin mixture (PSM) through a simple, repeatable and controllable separation method from alfalfa. The compounds of the saponin mixture were identified as Azukisaponin V and Soyasaponin I, which was determined as 79.26 and 20.74% respectively by HPLC-evaporative light scattering detection (ELSD) method. The effects of PSM on plasma lipid metabolism were studied in hyperlipidemic mice induced by Triton WR-1339. Results showed that PSM could significantly inhibit the increase of total cholesterol (TC), triacylglycerol (TG) and low density lipoprotein cholesterol (LDL-C) levels, and the decrease of plasma high density lipoprotein cholesterol (HDL-C) level in plasma of hyperlipidemic mice induced by Triton WR-1339. Further study demonstrated that PSM not only enhanced the activities of lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) in liver, but also increased their mRNA levels in hyperlipidemic mice. The results above indicate that PSM from alfalfa exerts effects on plasma lipid metabolism through elevating the activity of liver lipases in hyperlipidemic mice. The obtained data would be a good contribution to the development and utilization of alfalfa in the world.

収録刊行物

• ＪＯＵＲＮＡＬ　ＯＦ　ＨＥＡＬＴＨ　ＳＣＩＥＮＣＥ

  ＪＯＵＲＮＡＬ　ＯＦ　ＨＥＡＬＴＨ　ＳＣＩＥＮＣＥ 57 (5), 401-405, 2011

  公益社団法人 日本薬学会

キーワード

• alfalfa
• saponin
• Triton WR-1339
• lipid metabolism

詳細情報

• CRID: 1390282679475089408
• NII論文ID: 130001151380
• NII書誌ID: AA11316464
• DOI: 10.1248/jhs.57.401
• ISSN: 13475207
• ISSN: 13449702
• NDL書誌ID: 11250077
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11250077
• Web Site: http://www.jstage.jst.go.jp/article/jhs/57/5/57_5_401/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012198321
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
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