Begonia×hiemalisの生長と分化に及ぼす各種支持体の影響

書誌事項

タイトル別名
  • Effect of Supporting Materials on the Growth and Regeneration of Begonia*hiemalis Cultured In Vitro.
  • Begonia hiemalis ノ セイチョウ ト ブンカ ニ オヨボス カ

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抄録

Shoots and petiole segments of Begonia × hiemalis were cultured in vitro in liquid MS medium containing various supporting materials. The plantlets cultured on vermiculite, polyester fiber, sand, and phenol resin grew well. Vigorous adventitious shoots formed on petiole segments, cultured on vermiculite and polyester fiber. The pH of media containing peat moss and rock wool changed from 4.0 to 3.6 and 7.3 to 8.5, respectively, after autoclaving. The liquid media supplemented with peat moss, sawdust, pulp, and phenol resin which contained high concentrations of phenolic substances turned brown after autoclaving. The phenolic substances suppressed the growth of plantlets and regeneration rates of adventitious shoots from petiole segments. Several supporting materials released or adsorbed PO43-, Ca2+, and Fe2+ to or from the media, but the process did not affect the growth of plantlets. Vermiculite was considered to be the most suitable supporting material, and an alternative to agar.

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 62 (4), 839-844, 1994

    一般社団法人 園芸学会

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