Comparison of Two Quantitative Assays for Xenotropic Murine Leukemia Virus-Related Virus

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  • SATO Eiji
    Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University
  • YOSHIKAWA Rokusuke
    Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University Graduate School of Human Environmental Studies, Kyoto University
  • MIYAZAWA Takayuki
    Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University

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Xenotropic murine leukemia virus-related virus (XMRV), a novel gammaretrovirus in humans, was found in patients with prostate cancer (PC) and chronic fatigue syndrome (CFS). However, there has been controversy whether XMRV is directly associated with human diseases. In this study, we developed a LacZ marker rescue assay using human embryonic kidney 293T cells and a focus assay using a feline fibroblastic sarcoma-positive leukemia-negative QN10S cells. XMRV induced prominent foci in QN10S cells and the viral titer determined by the focus assay was as high as that by the LacZ marker rescue assay. Because the focus assay is simple and sensitive, it will be useful for monitoring infectious XMRVs in CFS and PC patients and virological studies for XMRV.<br>

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