水稲種実のミオ‐イノシトールの生合成　ＩＶ　　水稲乳熟期種実中におけるミオ‐イノシトール‐１‐りん酸合成酵素の諸性質

• 早川 利郎

  新潟大学農学部農芸化学科

• 倉沢 文夫

  新潟大学農学部農芸化学科

書誌事項

タイトル別名

• Some Enzymatic Properties of Myo-Inositol 1-Phosphate Synthase on Milky Stage of Rice Seed
• スイトウ ニュウジュクキ シュジツチュウ ニ オケル ミオーイノシトールー1

抄録

The specific activity of myo-inositol 1-phosphate synthase in the milky stage of rice seed reached a maximum on 10 th to 14 th days after flowering. Myo-inositol 1-phosphate synthase was purified from milky stage rice seeds by ammonium sulfate fractionation, DEAE-cellulose column chromatography, gel filtration on Sephadex G-200 and electrophoresis. The specific activity at the final stage became 168 fold.<br> The optimum pH and temperature for the activity were 8.0 and 50°C, respectively. The activity remained 90 to 100% in a pH range between 7.5 to 9.0 after storage at 4°C for 24 hr and 30% of enzyme activity remained after the incubation at 70°C for 10min. The enzyme was stable for 6 weeks in a frozen state at -20°C.<br> The partially purified enzyme required NAD⁺ for its activity and NH₄⁺ enhanced the activity twice. The apparent Km value for glucose 6-phosphate was 1.44mm.

収録刊行物

• 日本農芸化学会誌

  日本農芸化学会誌 50 (8), 339-344, 1976

  公益社団法人 日本農芸化学会

キーワード

• Medicine (miscellaneous)
• Chemistry (miscellaneous)
• Food Science
• Biotechnology

詳細情報

• CRID: 1390282679510446720
• NII論文ID: 130001225465
• NII書誌ID: AN00196191
• DOI: 10.1271/nogeikagaku1924.50.8_339
• ISSN: 18836844
• ISSN: 00021407
• NDL書誌ID: 1725619
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I1725619
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可