アフィニティクロマトグラフィーによるリボフラビンシンターゼの精製

• 灘本 知憲

  滋賀県立短期大学食物学科

書誌事項

タイトル別名

• Purification of riboflavin synthase by affinity chromatography.
• アフィニティ クロマトグラフィー ニ ヨル リボフラビンシンターゼ ノ セイセ

抄録

Affinity chromatography with commercial AH-Sepharose 4B and an inhibitor as the ligand was used to purify riboflavin synthase. AH-Sepharose 4B was coupled with 6-carboxyethyl-7-hydroxy-8-ribityllumazine (an inhibitor of the enzyme reaction). The enzyme showed a specific affinity with the gel and could be eluted with 5m_M 6, 7-dimethy-8-ribityl lumazine (the substrate) in 0.1_M K₂HPO₄. The enzyme was purified 100-fold by chromatography, when the crude extract from Eremothecium ashbyii was used as the enzyme source. The specific activity (14, 600) of the purified enzyme indicates that the method can be used for purification of the enzyme.

収録刊行物

• 日本農芸化学会誌

  日本農芸化学会誌 62 (7), 1077-1079, 1988

  公益社団法人 日本農芸化学会

キーワード

• Medicine (miscellaneous)
• Chemistry (miscellaneous)
• Food Science
• Biotechnology

詳細情報

• CRID: 1390282679482925056
• NII論文ID: 130001229018
• NII書誌ID: AN00196191
• DOI: 10.1271/nogeikagaku1924.62.1077
• ISSN: 18836844
• ISSN: 00021407
• NDL書誌ID: 3185054
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3185054
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可