大腸菌リボゾームＲＮＡの合成と制御に関する研究

DOI Web Site PubMed

書誌事項

タイトル別名
  • Transcriptional Regulation of <i>Escherichia coli</i> Ribosomal RNA Synthesis <i>in vitro</i>
  • ダイチョウキン リボソーム RNA ノ ゴウセイ ト セイギョ ニカンスルケン

この論文をさがす

抄録

The chromosome of a transducing phage λrifd18 carries a set of rRNA genes of E. coli. I have studied in vitro requirements for the preferential transcription of rRNA using λrifd18 DNA as a template. The following results were obtained: (1) Addition of Rs factor purified from ribosome wash stimulated total RNA synthesis. (2) With crude RNA polymerase prepared from extracts of E. coli, 17S and 23S premature rRNAs were synthesized, comprising 50% of the total transcription. Addition of guanosine tetraphosphate (ppGpp) resulted in selective inhibition of the rRNA synthesis. (3) When purified RNA polymerase was used, 7S, 12S and 26S transcripts were synthesized by addition of termination factor ρ and Rs factor. The 7S and 12S transcripts are mRNAs, which were initiated from PR and PL promotors of λ phage genome. When ppGpp was added, the amounts of 7S and 12S RNA were increased, but that of 26S RNA was not significantly affected. By DNA-RNA hybridization analysis, 26S RNA contained mostly the 30S precursor rRNA species. (4) Fractionation of the S100 fraction was accomplished by two sequential chromatography and by gel filtration. Each fraction was added to the purified RNA polymerase-λrifd18 DNA system, and the amount of rRNA synthesized was determined by DNA-RNA hybridization. Addition of the purified S factor stimulated synthesis of rRNA, and the transcription is susceptible to ppGpp inhibition.<br>It is expected that the S factor detected in vitro plays a physiological role as a positive effector controlling the in vivo transcription of rRNA.

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ