好気性，グラム陽性，カタラーゼ陽性球菌の迅速同定キットの開発　Ｍｉｃｒｏｐｌａｔｅ　ｈｙｄｒｉｄｉｚａｔｉｏｎによる全被検菌株の予備同定を踏まえて

DOI Web Site PubMed 被引用文献4件
  • 河村 好章
    岐阜大学医学部微生物学講座 大阪市立大学医学部細菌学教室
  • 長瀬 弘司
    テルモ株式会社技術開発本部診断開発部門
  • 遠藤 正和
    テルモ株式会社技術開発本部診断開発部門
  • 重松 貴
    テルモ株式会社技術開発本部診断開発部門
  • 江崎 孝行
    岐阜大学医学部微生物学講座
  • 藪内 英子
    岐阜大学医学部微生物学講座 大阪市立大学医学部細菌学教室

書誌事項

タイトル別名
  • STAPHYOGRAM, a new rapid identification kit for the aerobic, Gram-positive, catalase-positive cocci. Application of fluorometric microplate hydridization for the pre-identification of 386 isolates used.
  • コウキセイ グラム ヨウセイ カタラーゼ ヨウセイ キュウキン ノ ジンソク
  • Application of fluorometric microplate hybridization for the pre-identification of 386 isolates used
  • Microplate hybridizationによる全被検菌株の予備同定を踏まえて

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抄録

A new simplified test kit, STAPHYOGRAM plate, was developed for 4-hr identification of aerobic, Gram-positive and catalase-positive cocci. The plate has 18 wells, in which different dehydrated substrates and nutrients are fixed. An 18-hr agar-culture suspension of a test strain with a turbidity of McFarland No.4 was distributed into all wells in 50-μl quantities. After 4-hr incubation at 37C, the profile number was obtained by summarizing positive reactions.<br>The ability of the plate to differentiate the type strains of the 30 species of the three genera in the family Micrococcaceae was confirmed. These three genera are Staphylococcus, Micrococcus and Stomatococcus. The applicability of the fluorometric microplate hybridization technique to identification of aerobic, Gram-positive and catalase-positive cocci was confirmed by homologous hybridization among the type strains of the 30 species. Thus, 386 isolates of human and animal origin were pre-identified by microplate hybridization and used for evaluating the STAPHYOGRAM plate. Of the 236 profile numbers thus obtained with the 386 isolates, 218 (92.4%) were speciesproper each and all for the 15 species of Staphylococcus and Stomatococcus mucilaginosus. A total of 342 (88.6%) of the 386 isolates were given such profile numbers, and were identified without any additional test. Among the 15 species identified primarily by the results of STAPHYOGRAM plate culture, S. caprae, S. lugdunensis, S. gallinarum and S. delphini were validly published after Approved Lists of Bacterial Names. The identified strains of S. caprae (48), S. haemolyticus (46), S. capitis (35) numbered between those of S. epidermidis (67) and S. saprophyticus (31). Profile numbers common to two species were seven (27 strains) and that to four species was one (17 strains). These 44 strains were identified with one to three additional tests.<br>From these results, we were convinced that the STAPHYOGRAM test plate is useful for the rapid identification of members of family Micrococcaceae. By compiling STAPHYOGRAM plate data on genetically identified strains, an exclusive list of profile numbers will soon be prepared for perfection of the kit.

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