Effect of RNA Interference of BID and BAX mRNAs on Apoptosis in Granulosa Cell-derived KGN Cells
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- SAI Takafumi
- Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
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- MATSUDA Fuko
- Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
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- GOTO Yasufumi
- Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
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- MAEDA Akihisa
- Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
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- SUGIMOTO Miki
- Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan
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- GAO Hong-Mei
- Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
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- KABIR Abul Khair Mohammad Ahan
- Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
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- LI Jun-You
- Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan
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- MANABE Noboru
- Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan Research Center for Food Safety, The University of Tokyo, Tokyo 113-8657, Japan
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抄録
In mitochondrion-dependent type II apoptosis, BH3-interacting domain death agonist (BID) and BCL-2-associated X protein (BAX) promote death ligand and receptor-mediated cell death. In porcine ovaries, the levels of BID and BAX increase in follicular granulosa cells during atresia. In the present study, to confirm the pro-apoptotic activity of BID and BAX in granulosa cells, we examined the effect of RNA interference of BID or BAX on apoptosis using a human ovarian granulosa tumor cell line, KGN. By reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, expression of BID and BAX was detected in KGN cells. Then, we suppressed BID and BAX mRNA expression in KGN cells using small interfering RNA (siRNA). When BID or BAX was suppressed, a significant decrease in the apoptotic cell rate was noted. In granulosa-derived cells, BID and BAX showed pro-apoptotic activity. These results suggest that BID and BAX act as signal-transducing factors in mitochondrion-dependent type II apoptosis.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 58 (1), 112-116, 2012
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681313710208
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- NII論文ID
- 10030407425
- 130001388599
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC38zntFalsQ%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 023510868
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- PubMed
- 22052011
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可