書誌事項
- タイトル別名
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- Stimulatory Effects of Phenytoin on Osteoblastic Differentiation in Cultured MC3T3-E1 Cells.
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抄録
Phenytoin (diphenylhydantoin, DPH), an anticonvulsant drug for epileptic patients, has several adverse effects that include calvarial thickening and coarsening of the facial features. These effects often occur with chronic DPH therapy. While previous studies have shown DPH to heve an anabolic action on bone cells in vivo and in vitro, the basis of these effects is not fully understood. In this study, the effect of DPH on osteoblastic differentiation in cultured MC 3 T 3-E 1 cells was investigated by measuring mineralized bone nodule (BN) formation, alkaline phosphatase (ALPase) activity, cell growth, and the expression of matrix proteins, such as osteocalcin (OCN), and osteopontin (OPN). Continuous treatment of MC 3 T 3-E 1 cells with DPH for 20 days increased the BNs (9.1 to 19.7-fold) in a dose-dependent manner for concentrations of 10-50 μM DPH. ALPase activity was also stimulated by DPH (1.4-3.2 hold) in a dose-dependent manner for concentrations of 10-100 μM. Although a concentration 50 μM DPH increased the DNA content of the cells on days 5, 10, and 15, the final cell saturation density was not affected by any concentration of DPH. When DPH was added at two different culture stages, days 1-10 (growth stage) and days 11-20 (matrix development stage), BN formation was markedly stimulated in the case of the latter addition. A maximal effect was observed at a concentration of 200 μM DPH. The expression of OCN and OPN mRNA was also augmented by continuous treatment at a concentration of 200 μM DPH on days 1-20, and a remarkable effect was also observed when DPH was added on days 11-20. These findings demonstrate that DPH stimulates osteoblastic markers, such as BNs, ALPase activity, and OCN and OPN expression, mainly during the stage of matrix development in MC 3 T 3-E 1 cells. J. Jpn. Soc. Periodontol., 42: 104-113, 2000.
収録刊行物
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- 日本歯周病学会会誌
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日本歯周病学会会誌 42 (2), 104-113, 2000
特定非営利活動法人 日本歯周病学会
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詳細情報 詳細情報について
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- CRID
- 1390282679387262848
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- NII論文ID
- 110004013170
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- NII書誌ID
- AN0019129X
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- ISSN
- 1880408X
- 03850110
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可