Analysis of Tumor Necrosis Factor Receptor 1 and 2 Gene Polymorphisms in Japanese Patients with Generalized Aggressive Periodontitis.

DOI 52 References
  • Shimada Yasuko
    Division of Periodontology, Department of Oral Biological Science
  • Tai Hideaki
    Division of Periodontology, Department of Oral Biological Science
  • Endo Motohiro
    Division of Periodontology, Department of Oral Biological Science
  • Kobayashi Tetsuo
    Division of Periodontology, Department of Oral Biological Science General Dentistry and Clinical Education Unit, Niigata University Dental Hospital
  • Yamazaki Kazuhisa
    Division of Periodontology, Department of Oral Biological Science
  • Yoshie Hiromasa
    Division of Periodontology, Department of Oral Biological Science

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  • ＴＮＦレセプター１型および２型遺伝子多型と広汎性侵襲性歯周炎の関連性について

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Abstract

Periodontitis is considered to be a polygenic disease. Susceptibility to aggressive periodontitis (AgP), formerly known as early-onset periodontitis, might be associated with genetic polymorphisms. Tumor necrosis factor receptor (TNFR) 1 and 2 are cell surface receptors for TNF-α. Recent studies have suggested that TNFRs may modulate TNF-α-mediated inflammatory responses in periodontal disease. The aim of the present study was to evaluate whether TNFR1 and TNFR2 gene polymorphisms are associated with AgP in Japanese patients.<BR>Forty-five generalized AgP (G-AgP) patients and 100 age-matched healthy Japanese subjects were selected according to established clinical criteria. Single nucleotide polymorphisms at positions -383 (A/C) and +36 (A/G) in the TNFR1 gene, at positions +587 (T/G) and +694 (G/A) in the TNFR2 gene, and the variable number of tandem repeats (VNTR) promoter polymorphism in the TNFR 2 gene were detected using polymerase chain reactions, restriction fragment length polymorphisms, single-strand conformation polymorphisms, and direct sequencing methods. Statistically significant differences between the AgP and healthy groups were determined using chi-square and Fisher exact tests, equivalent results were assessed using the equivalence-delta test and further adjusted using the Mantel-Haenszel method.<BR>The frequency of the -383C allele was significantly lower in the G-AgP patients group than in the healthy group (p=0.04). On the other hand, equivalent allele frequencies between the two groups were found at three positions (+587 (T/G), +694 (G/A) and VNTR) in the TNFR2 gene. After adjusting for a confounding factor, the +587 (T/G) and VNTR allele frequencies of the two groups were found to be significantly equivalent (p<0.01).<BR>These findings suggest that the TNFR1 (-383A/C) gene polymorphism might be associated with G-AgP, whereas the TNFR2 +587 (T/G) and VNTR gene polymorphisms are not likely to result in a susceptibility to G-AgP. J Jpn Soc Periodontol, 45: 267-278, 2003.

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