利尿剤に関する研究 (第3報)

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  • Pharmacological studies of diuretics (3)
  • シガイブ ケンシュツキ ツキ ジドウ エキタイ クロマトグラフィー ニ ヨル ケッチュウ ニョウサン ノ ブンリ テイリョウ
  • Measurement of uric acid in rat serum using the automatically record liquid chromatograms of ultraviolet ray absorption
  • 紫外部検出器付自動液体クロマトグラフィーによる血中尿酸の分離定量

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A new method for the measurement of uric acid in rat serum was studied. It involvus the isolation of uric acid by ion-exchange separation and automatically quantitative determination of uric acid in the elute by 287mμ absorption. The apparatus isJLC-2A-BC2 type of Japan electron optics laboratory co, LTD.. The condition of measure-ment is followed, stationary phase AG 50W×8 (200-400 mesh), mobil phase 0.5 N-HCL, flow rate 0.76 ml/min, separation column 0.8∅×50cm (35°C), paper speed 60 cm/hr.By this method the rat serum uric acid was measured authentically and continuously every 30 minutes without influence of coexistent substance. A series of determinationsof uric acid in rat serum compares well with determinations on the same samples using the colorimetric Eichhorn method. This method is seemed to be significant for the authentic measurement of serum uric acid.

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