コイ・ミオシンB超沈殿の濁度変化による測定

• 山田 典彦

  北海道大学水産学部生物化学教室

• 新井 健一

  北海道大学水産学部生物化学教室

書誌事項

タイトル別名

• An assay of "superprecipitation" of carp myosin B by the change in turbidity.
• コイ・ミオシンＢ超沈澱の濁度変化による測定
• コイ ミオシン B チョウ チンデン ノ ダクド ヘンカ ニ ヨル ソクテイ

抄録

A study was made on the superprecipitation of carp myosin B by measuring the change in turbidity induced by addition of ATP.<br> On addition of 0.2-0.4mm ATP, carp myosin B suspension in a medium containing 80-100mM KCl, 1mM MgCl₂, 0.1mM CaCl₂, 0.2-0.4mg/ml of myosin B, and 25mM Tris-maleate (pH 7.0), showed a typical change in turbidity at 25°C; that is, an early instant decrease for some time (clearing phase), followed by a rapid large increase leading to the maximum.<br> The turbidity changes, induced under above conditions, are favourable for estimation of superprecipitation of carp myosin B. The rate of superprecipitation was then demonstrated as the reciprocal value of the time required for half-maximal change in turbidity (t_1/2)^-1. It was thus found that (t_1/2)^-1 and turbidity change were in linear relations when the protein concentra-tion is lower than the approximate of 0.4mg/ml.

収録刊行物

• 日本水産学会誌

  日本水産学会誌 53 (1), 77-83, 1987

  公益社団法人 日本水産学会

キーワード

• Aquatic Science

詳細情報

• CRID: 1390001206412985856
• NII論文ID: 130001542857
• NII書誌ID: AN00193422
• DOI: 10.2331/suisan.53.77
• COI: 1:CAS:528:DyaL2sXhsFWrsbo%3D
• ISSN: 1349998X
• ISSN: 00215392
• ISSN: http://id.crossref.org/issn/00215392
• NDL書誌ID: 3121168
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010342559
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3121168
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可