魚類の筋肉コラーゲンの簡易定量法

• 佐藤 健司

  京都大学農学部水産学科

• 吉中 禮二

  京都大学農学部水産学科

• 佐藤 守

  京都大学農学部水産学科

• 池田 静徳

  京都大学農学部水産学科 Department of Fisheries, Faculty of Agriculture, Kinki University

書誌事項

タイトル別名

• A simplified method for determining collagen in fish muscle.
• 魚類の筋肉コラーゲンの簡易定量法〔英文〕
• ギョルイ ノ キンニク コラーゲン ノ カンイ テイリョウホウ エイブン

抄録

The muscle of carp was fractionated into water-soluble, alkali-soluble (0.1_N NaOH), acid-soluble (0.5M acetic acid), hot water-soluble (extraction with water at 120°C for 1h), and residual fractions. The analyses of amino acid and SDS-polyacrylamide gel electrophoresis showed that collagen in the muscle was quantitatively recovered only in the acid-soluble and hot water-soluble fractions and non-collagenous proteins were absent in these fractions. On the basis of the colori-metric determination of protein in these fractions, we devised a simplified method for determinating the soluble and insoluble collagens in the muscle.<br> The soluble and insoluble collagens in the muscles of the rainbow trout Salmo gairdnerii, Japanese mackerel Scomber japonicus, carp Cyprinus carpio, and eel Anguilla japonica were deter-mined by the proposed method. The total collagen contents in the muscles of rainbow trout, Japanese mackerel, carp, and eel were found to be 0.47, 0.50, 0.60, and 1.99% of the wet muscle, respectively.

収録刊行物

• 日本水産学会誌

  日本水産学会誌 52 (5), 889-893, 1986

  公益社団法人 日本水産学会

キーワード

• Aquatic Science

詳細情報

• CRID: 1390001206412447104
• NII論文ID: 130001542945
• NII書誌ID: AN00193422
• DOI: 10.2331/suisan.52.889
• COI: 1:CAS:528:DyaL28Xktlyksrc%3D
• ISSN: 1349998X
• ISSN: 00215392
• ISSN: http://id.crossref.org/issn/00215392
• NDL書誌ID: 3082597
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010330980
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3082597
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可