凍結貯蔵したコイミオシンBの熱失活からみた凍結変性の解析
書誌事項
- タイトル別名
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- An explanation of freeze denaturation on the kinetics of thermal inactivation of frozen-stored carp myosin B.
- 凍結貯蔵したコイミオシンのBの熱失活からみた凍結変性の解析
- トウケツ チョゾウシタ コイミオシン B ノ ネツ シッカツ カラ ミタ トウ
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The carp myosin B solution was frozen and stored at -3, -5, -10 and -20°C, in order to make clear the freeze denaturation of the protein caused by the concentrated KCI solution on the basis of the thermal inactivation mode of Ca-ATPase. The same manner was also carried out for the myosin B in 1.33M KCI solution under the unfreezing conditions of +3 and -5°C.<br> In the case of -3 and -20°C frozen storages, the thermal inactivation mode of Ca-ATPase changed from the single-phased first-order plots to the two-phased first-order plots after 11 and 3 days, respectively. In the case of -5 and -10°C frozen storages, the same phenomenon occurred after 6.5h; the apparent rate was larger at -10°C than at -5°C. The order of influence on storage temperature was -10°C>-5°C>-3°C, in the range-of-3 ?? -10°C. When the myosin B in 1.33M KCI solution was stored at-5°C (unfreezing condition), the two-phased first-order inactivation mode was found after 7h, and at +3°C after 26h. The single-phased first-order inactivation mode was restored on addition of carp F-actin to the myosin B which was changed to show the two-phased first-order one after frozen storage at -5°C for 12h and after unfrozen storage in 1.33M KCI solution at -5°C for 9h.<br> From these findings, we concluded that a portion of F-actin in carp myosin B which was frozen and stored in the range of -3 ?? -10°C, was denatured as a result of the exposure to con-centrated KCI solution; under this range, a lower temperature that is a higher KCI concentration had an accelerating effect on the denaturation of F-actin.
収録刊行物
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- 日本水産学会誌
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日本水産学会誌 52 (10), 1765-1770, 1986
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390001206411880192
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- NII論文ID
- 130001543086
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- NII書誌ID
- AN00193422
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- ISSN
- 1349998X
- 00215392
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- NDL書誌ID
- 3107659
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- IRDB
- NDL
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