魚類ミオシンＢより中性塩の存在下で解離するアクチンの定量

• 若目田 篤

  北海道大学水産学部生物化学教室

• 新井 健一

  北海道大学水産学部生物化学教室

書誌事項

タイトル別名

• The amount of actin portion dissociated from myosin B in the presence of NaCl.
• ギョルイ ミオシン B ヨリ チュウセイエン ノ ソンザイカ デ カイリスル

抄録

When myosin B was kept at 2-30°C in a medium containing 40mM Tris-maleate (pH7.0) and 0.3-2.0_M NaCl, soluble proteins in a medium of low ionic strength (50mM KCl, pH7.0) increased. SDS-polyacrylamide gel electrophoretograms exhibited that there was a large amount of actin in this soluble fraction. The relative amount (%) of soluble actin on the basis of actin content in native myosin B was then estimated by a staining density on the gel rod.<br>It was found that the amount of actin dissociated from myosin B increased as the treatment with NaCl proceeded. Thus, the maximum liberation of actin amounted to approximately 60% within 1-2h upon addition of 2_M NaCl (for carp) or 1_M NaCl (for white croaker). It was also found that the actin component in such a soluble form does not show activating effect on myosin Mg-ATPase nor inhibiting effect on myosin EDTA-ATPase.<br>Based on the above findings, we confirmed that actin portion of white croaker myosin B is more sensitive to NaCl compared to that of carp myosin B.

収録刊行物

• 日本水産学会誌

  日本水産学会誌 51 (3), 497-502, 1985

  公益社団法人 日本水産学会

キーワード

• Aquatic Science

詳細情報

• CRID: 1390282681388326912
• NII論文ID: 130001543342
• NII書誌ID: AN00193422
• DOI: 10.2331/suisan.51.497
• ISSN: 1349998X
• ISSN: 00215392
• NDL書誌ID: 3033603
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030303943
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3033603
• 本文言語コード: ja
• データソース種別:
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