<i>Vibrio</i> sp. AP-2の産生するβ-アガラーゼの精製と特性

書誌事項

タイトル別名
  • Purification and characterization of .BETA.-agarases from Vibrio sp. AP-2.
  • Vibrio sp.AP-2の産生するβ-アガラーゼの精製と特性〔英文〕
  • Vibrio sp.AP-2 ノ サンセイスル ベータ アガラーゼ ノ セイセ
  • Purification and characterization of β-agarases from Vibrio sp. AP-2

この論文をさがす

抄録

β-Agarases were purified from the culture fluid of a marine bacterium, Vibrio sp. AP-2, by am-monium sulfate precipitation, successive column chromatography, and nuclease treatment. The final enzyme preparations appeared to be homogeneous on polyacrylamide gel electrophoresis. The enzymes (agarases-a, -b, and-c) had molecular weight of 34, 000, 20, 000, and 18, 000 daltons, and the pH optimum of 6.5, 5.5, 7.0, respectively, and were stable in a pH region from 4.0 to 9.0, and at temperatures below 45°C. The agarases were β-agarase which degraded agar to yield neoagaro-oligosaccharides. Agarase-a and agarase-c hydrolyzed agar to give neoagarotetraose as the pre-dominant product, agarase-b gave neoagarobiose as the predominant product. The three enzymes did not react with κ-carrageenan.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 56 (5), 825-830, 1990

    公益社団法人 日本水産学会

被引用文献 (6)*注記

もっと見る

キーワード

詳細情報

問題の指摘

ページトップへ