Production of Clonal Ayu by Chromosome Manipulation and Confirmation by Isozyme Marker and Tissue Grafting
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- Han Hyon-Sob
- Department of Cultural Fisheries, Faculty of Agriculture, Kochi University
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- Taniguchi Nobuhiko
- Department of Cultural Fisheries, Faculty of Agriculture, Kochi University
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- Tsujimura Akio
- Wakayama Prefecture Freshwater Fisheries Center
Bibliographic Information
- Other Title
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- 染色体操作法によるアユのクローンの生産とアイソザイムマーカーおよび組織移植法によるその確認
- 染色体操作法によるアユのクローンの生産とアイソザイムマーカーおよび組織移植法によるその確認〔英文〕
- センショクタイ ソウサホウ ニ ヨル アユ ノ クローン ノ セイサン ト ア
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Abstract
We attempted to produce clonal fish by chromosome manipulation in ayu Plecoglossus altivelis. To induce mitotic gynogenesis diploids (mitotic-G2N as complete homozygous individuals), the eggs were inseminated with UV-irradiated sperm of normal diploid and were treated with a hy-drostatic pressure of 650kg/cm2 at 80 min after insemination and just before the first cell cleavage. The production of mitotic-G2N was confirmed by isozyme markers. The mitotic-G2N pro-duced from the female parents which were heterozygous at Gpi-1, Me-1 and Pgm loci showed segre-gation into two homozygous types at those loci, and they were female in all individuals. To pro-duce clonal fish, the eggs of mitotic-G2N were inseminated with the UV-irradiated sperm of normal diploid. The diploidization was achieved by retaining the second polar body with cold shock. Two families of clonal fish were produced and designated as clone-1 and clone-2. The clonal fish examined were all homozygous types in which their genotypes were the same as their mothers in each of clonal fish. The graft-tissue (a piece of operculum) transplanted into their intraclonal sibling hosts survived and was accepted. On the other hand, the graft-tissue trans-planted into the different clonal hosts was rejected in both clones. These results suggest that in-traclonal siblings possessed completely identical histocompatibility genes.
Journal
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- NIPPON SUISAN GAKKAISHI
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NIPPON SUISAN GAKKAISHI 57 (5), 825-832, 1991
The Japanese Society of Fisheries Science
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Keywords
Details 詳細情報について
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- CRID
- 1390001206412723840
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- NII Article ID
- 130001545315
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- NII Book ID
- AN00193422
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- ISSN
- 1349998X
- 00215392
- http://id.crossref.org/issn/09199268
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- NDL BIB ID
- 3723444
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed