<i>Chattonella marina</i> II型細胞表面に反応するモノクローナル抗体MR-21の認識する抗原決定基

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タイトル別名
  • An Epitope Recognized by the Monoclonal Antibody MR-21 which is Reactive with the Cell Surface of Chattonella marina Type II.
  • Chattonella marina2型細胞表面に反応するモノクローナル抗体MR-21の認識する抗原決定基〔英文〕
  • Chattonella marina 2ガタ サイボウ ヒョウメン ニ ハンノ

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抄録

The monoclonal antibody MR-21 was strongly reactive with the cells of Chattonella marina NIES-121 belonging to Type II. MR-21 reacted with several bands on the nitrocellulose sheet after the cell lysate was developed using the Western blotting method. Antigens were inactivated by periodate-oxidation, but not by heating, proteinase, nor enzymes hydrolyzing sugars. Electron microscopic observation of immunogold-stained ultrathin section of the cells revealed that the antigens were localized at the secretory vesicles and Golgi systems as well as on the cell surface. These results suggest that the antigen molecules which MR-21 recognizes may be glycoproteins, and the epitope may be in the carbohydrate side chains.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 57 (5), 885-890, 1991

    公益社団法人 日本水産学会

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