マコンブプロトプラストの単離への<i>Alteromonas</i> sp.産生アルギン酸リアーゼの利用
書誌事項
- タイトル別名
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- Application of an Alginate Lyase from Alteromonas sp. for Isolation of Protoplasts from a Brown Algae Laminaria japonica.
- マコンブプロトプラストの単離へのAlteromonas sp.産生アルギン酸リアーゼの利用〔英文〕
- マコンブ プロトプラスト ノ タンリ エ ノ Alteromonas sp.サ
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An alginate lyase from Alteromonas sp. H-4 was used for the preparation of protoplast from young sporophytes of Laminaria japonica. Optimum conditions for the isolation of protoplasts were as follows: a) fine pieces of the sporophytes chopped by a razor were incubated in an enzyme mixture for 3 to 5h at 15°C with shaking, b) the composition of the enzyme mixture was purified alginate lyase (30U/ml) with cellulase Onozuka R-10 (1.5%) in a hypertonic solution (0.7M mannitol, 25mM MgCl2, and 5mM HEPES in 50% seawater, pH7.8). Yields of protoplast were 106-107 cells/g (sporophytes) and protoplast viability was more than 75%. Protoplasts were brown-greenish and spherical with a diameter of 10-25μm. The purity of alginate lyase from the strain H-4 scarcely influenced the yield of protoplast, but purified enzyme produced the highest viability of cells.
収録刊行物
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- 日本水産学会誌
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日本水産学会誌 59 (4), 705-709, 1993
公益社団法人 日本水産学会
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詳細情報
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- CRID
- 1390001206411162368
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- NII論文ID
- 130001545872
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- NII書誌ID
- AN00193422
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- ISSN
- 1349998X
- 00215392
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- HANDLE
- 2115/52098
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- NDL書誌ID
- 3829557
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
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- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可