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タイトル別名
  • Lysozyme Activity in Shellfishes
  • カイルイ ノ リゾチーム ブンプ

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Lysozyme distributions in the shellfishes Tapes philippinarum, Meretrix lusoria, Corbiculina leana, Crassostrea gigas, Nordotis discus, scapharca broughtnii, Mytilus edulis, Anodonta japonica, Cristaria plicata, Charonia sauliae and Batillus cornutus were investigated.<br> Lysozyme was chromatographed on a chitin-coated cellulose column. The enzyme activity was assayed with Micrococcus lysodeikticus cells and by the ferricyanide method with glycol chitin as substrate.<br> Lysozyme was found in the liver and muscle of 5 shellfish species and in the gill and mucus of 4 species. High lysozyme activity was found in the whole body of M. edulis and C. plicata. The C. leana lysozyme showed maximum activity at pH 4.5 and 65°C. The enzyme remained active for 30 min up to 90°C at pH 4.8. At temperatures above 90°C, the enzyme was completely inactive. The activity of C. leana lysozyme was inhibited by incubation with Fe2+, Fe3+, Mn2+, Hg2+, Cd2+, Cu2+ and Ni2+ but slightly enhanced by Mg2+, Ba2+ and Zn2+.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 49 (1), 131-135, 1983

    公益社団法人 日本水産学会

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