ホタテガイ閉殻筋無紋筋部からのトロポミオシンの単離と性状

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書誌事項

タイトル別名
  • Isolation and characterization of scallop smooth adductor tropomyosin.
  • ホタテガイ閉殻筋無紋筋部からのトロポミオシンの単離と性状〔英文〕
  • ホタテガイ ヘイカク キン ムモン キン ブ カラ ノ トロポミオシン ノ タ

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抄録

Tropomyosin was isolated in a high yield, from smooth adductor of the scallop Patinopecten yessoensis by the method which was consisted essentially of isoelectric precipitation and DEAE Sephadex A-50 column chromatography. Scallop tropomyosin thus purified was homogeneous in SDS-gel electrophoretic and ultracentrifugal analyses.<br> Sedimentation constant (s020, w) of this tropomyosin was 2.63 S. The molecular weight was estimated to be 70, 000 by sedimentation equilibrium method, and the subunit molecular weight 36, 000 by SDS-gel electrophoresis, both these data indicating that scallop tropomyosin consists of two subunits of the same molecular weight.<br> The intrinsic viscosity [η] as measured in 1 M NaCl was 0.35dl/g. The salting-out range with ammonium sulfate of this protein was found between 38-44% saturation. Scallop tropomyosin exhibited an absorption spectrunm showing a maximum at 277 nm and a minimum at 252 nm. It was featured by high glutamic and aspartic acid contents, low histidine content, lack of tryptophan, etc.<br> In most of the above respects, scallop smooth adductor tropmyosin was recognized to be comparable to tropomyosins from various sources, especially from invertebrates.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 46 (9), 1183-1188, 1980

    公益社団法人 日本水産学会

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