脂質分子膜で被覆したＨｂ小胞体の調製条件

DOI

書誌事項

タイトル別名
  • Preparation conditions of human hemoglobin-vesicles covered with lipid membranes.

抄録

The quality of artificial red cells (ARC); Hb-vesicles (200nmφ), can be evaluated from the lamellarity (n) of Hb-vesicles and the concentration of Hb in lipid membrane vesicles ([Hb]in) as well as oxygen binding properties of Hb vesicles. It is important to prepare Hb-vesicles with higher concentration of Hb ([Hb]in) and lower lamellarity (n). [Hb]in increased with the concentration of Hb used for the preparation of Hb-vesicles. The Hb-vesicles with smaller lamellarity could be obtained with lowering the preparation temperature. Covering of lipid membrane to the concentrated Hb solution was also influenced by the ionic strength and the pH of the Hb solution because lipids and Hb are electrolytes. The highest [Hb]/[Lipid] ratio was obtained when the pH was at around isoelectric point of Hb (pI=7.0). The lamellarity decreased with decreasing the concentration of NaCl.

収録刊行物

  • 人工臓器

    人工臓器 22 (2), 566-569, 1993

    一般社団法人 日本人工臓器学会

キーワード

詳細情報 詳細情報について

  • CRID
    1390001204956142720
  • NII論文ID
    130001732745
  • DOI
    10.11392/jsao1972.22.566
  • ISSN
    18836097
    03000818
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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