Immunohistochemical Examination of Novel Rat Monoclonal Antibodies against Mouse and Human Podoplanin
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- Kaji Chiaki
- Department of Morphological Biology, Fukuoka Dental College Department of Morphological Biology, Fukuoka Dental College
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- Tsujimoto Yuta
- Molecular Tumor Marker Research Team, Yamagata University Global COE Program, Yamagata University Faculty of Medicine Molecular Tumor Marker Research Team, Yamagata University Global COE Program, Yamagata University Faculty of Medicine
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- Kato Kaneko Mika
- Molecular Tumor Marker Research Team, Yamagata University Global COE Program, Yamagata University Faculty of Medicine Molecular Tumor Marker Research Team, Yamagata University Global COE Program, Yamagata University Faculty of Medicine
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- Kato Yukinari
- Molecular Tumor Marker Research Team, Yamagata University Global COE Program, Yamagata University Faculty of Medicine Molecular Tumor Marker Research Team, Yamagata University Global COE Program, Yamagata University Faculty of Medicine
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- Sawa Yoshihiko
- Department of Morphological Biology, Fukuoka Dental College Department of Morphological Biology, Fukuoka Dental College
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抄録
This study aims to develop new monoclonal antibodies (mAbs) against mouse and human podoplanin. Rats were immunized with synthetic peptides, corresponding to amino acids 38–51 of mouse podoplanin or human podoplanin which is 100% homologous to the same site of monkey podoplanin; anti-mouse podoplanin mAb PMab-1 (IgG2a) and anti-human mAb NZ-1.2 (IgG2a) were established. In immunocytochemistry, the mouse melanoma B16-F10 and mouse podoplanin (mPDPN)-expressed CHO transfectant were stained by PMab-1; human lymphatic endothelial cells (LEC) and human podoplanin (hPDPN)-expressed squamous cell carcinoma HSC3 transfectant, were stained by NZ-1.2. Western-blot analysis detected an about 40-kDa protein in CHO-mPDPN and B16-F10 by PMab-1, and in HSC3-hPDPN and LEC by NZ-1.2. In frozen sections, PMab-1 reacted with mouse kidney, pulmonary alveoli, pulmonary pleura, and salivary gland myoepithelial cells while NZ-1.2 reacted to the human salivary gland myoepithelial cells. The immunostaining of paraffin-embedded sections also showed the reaction of PMab-1 or NZ-1.2 to the mouse or monkey kidney glomerulus, pulmonary alveoli, and lung lymphatic vessels. These results indicate that the two novel rat mAbs to the mouse and human/monkey podoplanin are useful for Western-blot and immunostaining of somatic tissues on paraffin-embedded sections as well as frozen sections.<br>
収録刊行物
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- Ａｃｔａ Ｈｉｓｔｏｃｈｅｍｉｃａ ｅｔ Ｃｙｔｏｃｈｅｍｉｃａ
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Ａｃｔａ Ｈｉｓｔｏｃｈｅｍｉｃａ ｅｔ Ｃｙｔｏｃｈｅｍｉｃａ 45 (4), 227-237, 2012
日本組織細胞化学会