Extraction of High-Quality RNA from Rubber Tree Leaves

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  • DENG Liu-Hong
    Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences
  • LUO Ming-Wu
    College of Materials Science and Chemical Engineering, Hainan University College of Materials Science and Chemical Engineering, Hainan University
  • ZHANG Chun-Fa
    Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences
  • ZENG Hui-Cai
    Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences

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A specific technique capable of producing high-quality RNA for rapid amplification of cDNA ends (RACE) was established for challenging tissues: leaves of the rubber tree. Total RNA was extracted by cetyltrimethylammonium bromide (CTAB)-LiCl combined with TRIzol reagent. The isolated RNA was highly intact. With RNA as template, full-length cDNA was obtained (NCBI, AY461413) by RACE.

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