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- 王子田 彰夫
- 九州大学大学院薬学研究院
書誌事項
- タイトル別名
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- Development of Specific Protein Labeling Method by Using Small Molecular Probes
- ショウブンシ プローブ ニ ヨル タンパクシツ ノ トクイテキ ラベルカ ギジュツ ノ カイハツ
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Selective protein labeling with a small molecular probe is a versatile method for elucidating protein functions in living cells. In this review, we report a new protein labeling method that enables selective covalent modification of a tag-fused protein with small functional molecules. This method utilizes the specific interaction and rapid reaction between a short peptide tag and a molecular probe, which comprises the cysteine-containing short CA6D4x2 tag (CAAAAAADDDDGDDDD) and a tetranuclear Zn(II)-DpaTyr probe containing a reactive α-chloroacetyl moiety. This labeling system, so-called reactive tag system, was successfully applied to the fluorescence imaging of tag-fused GPCRs such as bradykinin receptor (B2R) and acetylcholine receptor (m1AchR) expressing on HEK293 cells. The utility of this labeling method was demonstrated in the function analyses of GPCRs, such as fluorescence visualization of the stimuli-responsive internalization of GPCRs and pH change in endosomes containing the internalized GPCRs.<br>
収録刊行物
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- 薬学雑誌
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薬学雑誌 132 (2), 195-199, 2012-02-01
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282681104066432
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- NII論文ID
- 130001872008
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- NII書誌ID
- AN00284903
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- ISSN
- 13475231
- 00316903
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- NDL書誌ID
- 023511052
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 使用不可